Team capstone · 2026

Pocket-guided molecular docking

A team project testing whether predicted protein-binding pockets can guide docking toward more reliable ligand poses.

Timeline
May–July 2026
Setting
CMU Pre-College Computational Biology · team capstone
Tools
Python, PrankWeb, AutoDock Vina

The question

Can pocket predictions narrow the search?

Blind docking searches broadly across a protein. Our team asked whether predicting likely binding pockets first could improve the chance of recovering a successful pose.

The pipeline

Predict, dock, compare

We built a Python pipeline that used PrankWeb to predict pockets and AutoDock Vina to compare pocket-guided docking with blind docking. We benchmarked 97 protein–ligand complexes, then used 50 Astex complexes as a second evaluation set.

Team results

The figures below describe the team pipeline and benchmark, not individual performance.

Pocket-guided vs. blind docking

97-complex benchmark

In our team’s 97-complex benchmark, pocket-guided docking recovered successful poses in 56% of cases, compared with 35% for blind docking, and rescued 27 cases.

Astex evaluation

50 complexes

In our team’s 50-complex Astex evaluation, the median RMSD was 1.36 Å, and 62% of complexes were under the 2 Å threshold.

Sources & context

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